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Flow cytometric analysis of Jurkat cells staining with Monoclonal Anti-HLA class I Antibody, Human IgG1 (W632) (Cat. No. HLI-M597) at 0.3ug/mL(0.03ug corresponds to labeling of 1e6 cells in a final volume of 100 µL) , compared with isotype control antibody, washed and then followed by PE anti-human IgG Fc Recombinant Antibody and analyzed with FACS (QC tested).
Flow cytometric analysis of Jurkat cells staining with PE-Labeled Monoclonal Anti-HLA class I Antibody, Human IgG1 (W632) (Cat. No. HLI-CFM597) at 1:50 dilution(2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL) , compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).
Immobilized Biotinylated Anti-HLA class I Antibody, Human IgG1 (W632) (Cat. No. HLI-BM597) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human HLA-A*11:01&B2M&KRASG12V (VVGAVGVGK) Complex Protein (Cat. No. HLV-H52H3) with a linear range of 4-63 ng/mL (QC tested).
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